NMR relaxation and the structure of a synthetic DNA poly(dA-dT).poly(dA-dT).
نویسندگان
چکیده
1H-1H and 31P-1H nuclear Overhauser effects and 31P NMR spin-lattice relaxation times were measured for a synthetic DNA poly(dA-dT).poly(dA-dT) in a low-salt aqueous solution. The results have shown that all bases in the double helix are anti-orientated with respect to deoxyribose residues and that the sugar-phosphate backbone has an alternating architecture.
منابع مشابه
Two distinct conformers coexist in a synthetic DNA poly(dA-dT).Poly(dA-dT) in low-salt aqueous solution.
Circular dichroism spectroscopy (CD) sensitively reflects even slight alterations of the base stacking geometry in DNA (Tinoco et al. 1980; Johnson et al. 1981). However, the alterations cannot be unambiguously interpreted in conforma tional terms so that, for example, the question concerning the origin of the relatively extensive temperature-induced changes in the CD spectrum of a synthetic D...
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It has previously been demonstrated by other workers that the duplex of a synthetic DNA poly(amino2dA-dT) undergoes a salt-induced conformational isomerization. We show in the present work using circular dichroism that the same isomerization is induced in poly(amino2dA-dT) by various alcohols. The isomerization was originally identified as the B-to-Z and then B-to-A conformational transition of...
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BACKGROUND Base dependent binding of the cytotoxic alkaloid harmalol to four synthetic polynucleotides, poly(dA).poly(dT), poly(dA-dT).poly(dA-dT), poly(dG).poly(dC) and poly(dG-dC).poly(dG-dC) was examined by various photophysical and calorimetric studies, and molecular docking. METHODOLOGY/PRINCIPAL FINDINGS Binding data obtained from absorbance according to neighbor exclusion model indicat...
متن کاملThe structure of poly(dA):poly(dT) in a condensed state and in solution.
New X-ray and energetically optimal models of poly(dA):poly(dT) with the hydration spine in the minor groove have been compared with the NMR data in solution (Behling, R.W. and Kearns, D.R. (1986) Biochemistry 25, 3335-3346). These models have been refined to achieve a better fit with the NMR data. The obtained results suggest that the poly(dA):poly(dT) structure in a condensed state is similar...
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Polarized and depolarized Raman spectra are obtained for a number of synthetic polynucleotides containing adenine, uracil, and thymine bases. The depolarization ratios are determined by two methods: (1) by dividing the 1-spectrum by the 11-spectrum and ( 2 ) after curve fitting. Overlapping bands, isotope splitting, reorientational broadening, and noncoincidence splitting affect the magnitude o...
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ورودعنوان ژورنال:
- General physiology and biophysics
دوره 5 1 شماره
صفحات -
تاریخ انتشار 1986